Chem. Pharm. Bull.

Chem. Pharm. Bull., 53(7),843-846, July 2005

Notes

Synthesis and Triplex-Forming Ability of 2′,4′-BNAs Bearing Imidazoles as a Nucleobase

Yoshiyuki HARI,^a Satoshi OBIKA,^a,b Hiroyasu INOHARA,^a Masahiro IKEJIRI,^a Daisuke UNE,^a and Takeshi IMANISHI^*^,a

^a Graduate School of Pharmaceutical Sciences, Osaka University; 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan: and ^b PRESTO, JST; 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. * To whom correspondence should be addressed. e-mail: imanishi@phs.osaka-u.ac.jp

To expand the sequence of double-stranded DNA (dsDNA) targets in a triplex formation, 2′,4′-BNAs (2′-O,4′-C-methylene bridged nucleic acids) having imidazoles as a nucleobase were synthesized and incorporated into oligonucleotides. Triplex-forming ability of the modified oligonucleotides was evaluated by using melting temperature (T_m) measurements.

Key words methylene bridged nucleic acid (BNA); locked nucleic acid (LNA); triplex; imidazole; oligonucleotide

Notes

Synthesis and Triplex-Forming Ability of 2′,4′-BNAs Bearing Imidazoles as a Nucleobase

Yoshiyuki HARI,a Satoshi OBIKA,a,b Hiroyasu INOHARA,a Masahiro IKEJIRI,a Daisuke UNE,a and Takeshi IMANISHI*,a

a Graduate School of Pharmaceutical Sciences, Osaka University; 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan: and b PRESTO, JST; 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. * To whom correspondence should be addressed. e-mail: imanishi@phs.osaka-u.ac.jp

Yoshiyuki HARI,^a Satoshi OBIKA,^a,b Hiroyasu INOHARA,^a Masahiro IKEJIRI,^a Daisuke UNE,^a and Takeshi IMANISHI^*^,a

^a Graduate School of Pharmaceutical Sciences, Osaka University; 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan: and ^b PRESTO, JST; 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. * To whom correspondence should be addressed. e-mail: imanishi@phs.osaka-u.ac.jp