Chem. Pharm. Bull., 53(7),863-865, July 2005

Communications to the Editor

Synthesis of DNA Conjugate by Mechanochemical Solid-State Polymerization and Its Affinity Separation of Oligonucleotides Having Single-Base Difference by Capillary Electrophoresis

Shin-ichi KONDO,^* Daisuke SHICHIJYOU, Yasushi SASAI, Yukinori YAMAUCHI, and Masayuki KUZUYA

Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University; 5-6-1 Mitahora-Higashi, Gifu 502-8585, Japan. * To whom correspondence should be addressed. e-mail: skondo@gifu-pu.ac.jp

In this communication, we discuss the characterization of DNA conjugate synthesized by mechanochemical polymerization, Con-M, on the separation of model oligo-DNA and its single nucleotide polymorphisms (SNPs) by affinity capillary electrophoresis, compared with that prepared by radical-initiated solution polymerization, Con-RL. The average molecular weight of Con-M was similar to that of Con-RL, although the molecular weight distribution of Con-M was narrower than that of Con-RL. Capillary electrophoresis of oligo-DNA was performed using the capillary filled with DNA conjugate. The resolution of the capillary filled with Con-M was apparently higher than with Con-RL. It is considered that higher resolution using the capillary filled with Con-M could be ascribed not only to the narrow molecular weight distribution but also to the difference of copolymer structure.

Key words DNA conjugate; mechanochemical polymerization; affinity capillary electrophoresis; single nucleotide polymorphism; copolymer structure